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ABSTRACT
A 14-day laboratory investigation was carried out on the influence of the antibiotics: Tetracycline and Chloramphenicol, on the growth of microalgae Chlorella vulgaris and Scenedesmus obliquus. Concentrations of the Tetracycline-Chloramphenicol solution, spanning 0% to 25%, were utilized. Microalgae growth was assessed at 750nm on a visible a spectrophotometer, and samples were analyzed for physicochemical parameters every two days. The analysis incorporated descriptive statistics, inferential techniques including One-way ANOVA, paired t-tests, and Tukey test, executed using Microsoft Excel and SPSS 20. For Chlorella vulgaris, growth was evident only on days 0 and 2 for all concentrations. Likewise, Scenedesmus obliquus which experienced growth solely on days 0 and 2 across all concentrations, except at 25%, where there was steady growth. Notably, Scenedesmus obliquus displayed high binhibition percentages in all concentrations except 25%, whereas Chlorella vulgaris exhibited peak inhibition percentages at 1% and 5% concentrations. Throughout the experiment, both microalgae sustained temperatures within the optimal range of 20°C to 30°C. Their relatively low Dissolved Oxygen levels further contributed to growth suppression. As the experiment concluded, turbidity levels escalated, coinciding with elevated Total Dissolved Solids levels from days 10 to 14. These combined factors hindered growth by obstructing sunlight necessary for photosynthesis. The study validates that the presence of Tetracycline and Chloramphenicol solutions hampers microalgae growth due to these factors, alongside the phytotoxic nature of other organisms within the antibiotic solution.
