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ABSTRACT
The specific objectives of this study are to investigate the effect of montelukast and hydrocortisone on the histology of the kidney and on the fractional excretion of sodium and urea in asthma-induced Sprague-Dawley rats. The aim of this study is to investigate and identify the effects of montelukast and hydrocortisone on the histology of the kidney, fractional excretion of sodium and urea in asthma-induced sprague-dawley rats. To achieve this, 20 female Sprague-Dawley rats were used for this study which were divided into 2 main groups (control (GROUP 1) and test groups). Test group was subdivided into: GROUP 2: Asthmatic but not treated, GROUP 3: Asthmatic and treated with montelukast and GROUP 4: Asthmatic and treated with hydrocortisone, with 5 rats in each group. Control group were sensitized and challenged with intraperitoneal injection and aerosolized saline respectively. Asthma was induced by sensitizing the rats using 1 mg OVA (ovalbumin) and 200 mg aluminum hydroxide dissolved in 0.9 saline on day 0 and 7, and challenged with OVA (1 % w/v, adsorbed in 0.9 saline) twice weekly from day 7 of treatment until the last day using Medel family nebulizer with aerosol delivery of 0.28 ml/min. During the period of challenging, treatment began with 10 mg/kg Montelukast and 5 mg/kg hydrocortisone after confirmation of asthma in all test groups. Urine samples were collected and frozen for later urine electrolyte analysis. At the end of the experiment, all animals were euthanized and kidney tissue samples were collected for histological analysis; washed with normal saline, immersed in 10% (v/v) formaldehyde solution, and embedded in paraffin. Data obtained were expressed as mean ± Standard Error of Mean ( SEM) . Statistical analysis performed by one way analysis of variance (ANOVA) for assessing differences amongst multiple groups, followed by Tukey's test using Graphpad Prism 10.0.3 software (Graphpad, San Diego, CA). Results showed no significant difference on the fractional excretion of sodium and urea and no alterations on the histology of the kidney after treatment with montelukast and hydrocortisone compared to control. In conclusion, this study shows that the administration of montelukast and hydrocortisone has no significant effect on the fractional excretion of sodium and urea and no observable changes on the histology of the kidney.
