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ABSTRACT
The effect of Chlorpyrifos on the embryonic development of Clarias gariepinus from fertilization to cleavage was investigated using static-renewal assay. The experiment was carried out using embryo of Clarias gariepinus and these were exposed to varying concentrations of 0, 0.01, 0.10, and 10.0µg/l of chlorpyrifos in different experimental tanks. This was done to determine the morphological effects on the embryos. Results showed that there were significant morphological changes in surviving embryo in some of the concentrations. However, increase in concentration of Chlorpyrifos resulted in the death of embryo especially at 10.0µg/l. Hatchability rate decrease with increasing concentration of chlorpyrifos. The physiochemical parameter of the test media, such as temperature, pH, turbidity, total alkalinity, dissolved oxygen, water hardness and electrical conductivity was also analysed using standard methods. There was no significant difference in the water parameters both in the control and treatment groups. It is therefore recommended that the use of chlorpyrifos for vector control campaigns as well as for agricultural practices should be monitored closely to prevent runoffs of these substances into the aquatic ecosystem leading to massive decline in wild population of fish as a result of reproductive failure thereby upsetting the ecological balance in the aquatic ecosystem.