ABSTRACT

This study was conducted in order to determine the effect of different levels of pH, different carbon sources and culture media on the vegetative growth of Pleurotus djamor. Five millimeter (5 mm) inoculum block was taken from the growing edge of a pure culture of Pleurotus djamor. This was inoculated in a Potato Dextrose Agar medium with different pH levels (3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0). For carbon sources, a basal medium was prepared from glucose, fructose, sucrose, maltose, PDA and cellulose. For the culture media, PDA was prepared which serves as control. Highest mycelia extension for pH levels was at pH 6 with a value of (56.33±2.25 mm) and lowest in pH 7 with a value of 47.14±1.6 mm. Highest mycelia extension for carbon source was observed in fructose with a value of (51.33±5.62 mm) and lowest extension in cellulose with a value of (37.33±3.88 mm). SDA (Sorghum Dextrose agar) was observed to support the highest spread of mycelia of 56.83±1.04 mm and RDA (Rice Dextrose agar) with the lowest mycelial spead for culture media. Formulated PDA (FPDA) and synthethic PDA has the highest mycelial density of (5+) with abundant biomass of 0.12±0.39 g and 0.18±0.30 respectively, for culture media. The mycelial density rating for pH 5.0, 6.0 and 8.0 have the highest densities of (5+) each. For carbon sources Potato Dextrose agar has the highest mycelial density of (5+) with 0.124±0.02g biomass value. Sucrose, maltose and cellulose have the least mycelial density with (1+) with 0.125±0.02g, 0.09±0.07g and 0.13±0.04g biomass values respectively. In this study, it was observed that growth rate was high in yam dextrose agar, sorghum dextrose agar and cocoyam dextrose agar but low in rice dextrose agar. This study showed that various media pH and carbon growth of Pleurotus djamor.