ABSTRACT
Disease associated with recreational centers remain a major problem due to contamination of the pool water. The study aimed at investigating the efficacy of water treatment against microbial and physico-chemical parameters of swimming pool in Delta Central Senatorial district considering the great significance of the safety of recreational water.
A total of 108 samples were obtained from three pools before and after use from three hotels at Delta Central Senatorial district using sterile bottles with capacity of 1liter intended for three months. Samples were conveyed to the research center for analyses using usual techniques to assess for physico-chemical parameters and membrane filter technique for microbial parameters. The results obtained showed that physico-chemical properties were within World Health Organization and Environmental Protection Agency recommended values before use with variation among Sites 1, 2, and 3 while there was a slight deviation from the recommended values after use. The mean bacterial count between 0.00cfu -2cfu before use and 0.00cfu-214 CFU/100ml after use.
Bacterial isolates were susceptible to calcium hypochlorite concentration at 0-5ppm, 6-10ppm and 11-15ppm except Staphylococcus aureus which ensued at Site 2 before use during week 2. All the bacterial isolates remained resistant to amoxicillin 30µg, ampiclox 30µg, erythromycin 10µg streptomycin 30µg, and septrin 30µg while E.coli and Proteus vulgaris were additionally resistant to tarivid 10µg and sparfloxacin 10µg.
All bacteria recovered were susceptible to gentamycin 10µg, ciprofloxacin 10µg, pefloxacin 10µg, rocephin 25µg, zinnacef 25µg and augmentin 30µg, and Candida albicans which was susceptible to all antibiotics. After plasmid curing E.coli and Proteus vulgaris harboring plasmid became susceptible to all the antibiotics they were initially resistant to. However, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis that recorded resistance did not habour any plasmid.
The study findings suggested that the observed problem can be reduced by promoting good cleanness sensitization, sanitizing of the pool and enforcement of recommended guidelines at regular intervals to ensure a proper control of microbial and chemical conditions.
