ABSTRACT
The study delves into the impact of Montelukast and hydrocortisone on renal function markers— electrolytes (na, k, hco3, and cl), urea, and creatinine—in asthma-induced Sprague Dawley rats. The aim of this study is to evaluate the effect of montelukast and hydrocortisone on some renal function markers in asthma induced Sprague Dawley rats. Sprague-Dawley rats weighing between 180-250 g were divided into two (2) main groups; the Control group and Test group. The test group were further divided into three (3) subgroups in which one group consists of asthmatic rats which were not treated with anti-asthmatic drugs and the other two groups consists of asthmatic rats treated with anti-asthmatic drugs (Montelukast and Hydrocortisone). All the groups consisted of twenty (20) rats each (n=5). The control group received normal rat chow and water throughout the experimental period while the test groups were sensitized with 1 mg OVA and 200 mg aluminum hydroxide dissolved in 0.9 saline on day 0 and 7, challenged with OVA (1 % w/v, adsorbed in 0.9 saline) twice weekly from day 7 of treatment until the last day, and were treated with 5 mg/kg hydrocortisone (i.p) and 10 mg/kg Montelukast for 28 days. At the end of the treatment period, all animals were euthanized. Blood was collected from abdominal aorta of rats and serum was isolated by aspiration. The separated serum was stored at frozen for the later quantitative determination of some biomarkers. The concentration of electrolytes (sodium, potassium, bicarbonate and chloride), urea and creatinine in the serum samples were measured using flame photometry. The results of the study were analyzed statistically using a one-way analysis of variance (ANOVA) for assessing differences amongst multiple groups, followed by Tukey's test using Graphpad Prism 10.0.3 software. P < 0.05 was considered statistically significant. The results of this study reveal that there were no statistically significant differences in the concentrations of sodium, potassium, bicarbonate, chloride, urea, and creatinine when compared to the control group (p > 0.05). Moreover, the comparisons against the negative control and montelukast groups also did not yield any statistically significant differences (αp<0.05 and ϕp<0.05, respectively). The lack of statistically significant differences in renal function markers suggests that both montelukast and hydrocortisone, when administered to asthma-induced Sprague Dawley rats, did not significantly alter the evaluated parameters. This could indicate that these medications might not adversely affect renal function in the short-term as observed in this study.
