EFFECT OF KOLAVIRON ON SOME INFLAMMATORY MARKERS AND ANTIOXIDANT ENZYMES OF WISTAR RATS FED WITH HIGH FAT DIET.

ABSTRACT

Kolaviron (KV) is a fraction of the defatted acetone extract of Garcinia kola seeds. It has been reported to have both antioxidative and anti-inflammatory effects. The aim of this study is to investigate the effect of kolaviron on some inflammatory makers and antioxidant enzymes of rats fed with high fat diet. 29 adult male Wistar rats, weighing about 115–158g were used for this study. The initial weight of the animals were measured and recorded. The animals were randomly assigned into three groups with five animals in group A and 8 in group B and C respectively. Animals in group A served as control and were fed with normal feed for 6weeks. Group B were fed with high fat diet only for 6 weeks. While Group C were fed with high fat diet for a period of 6 weeks and then administered with 0.4ml of kolaviron for another 14 days. Blood samples were collected by cardiac puncture in lithium heparin bottles and EDTA bottles for assay of inflammatory makers, antioxidative enzymes and lipid profile. The serum levels of triglyceride, total cholesterol and high density lipoprotein (HDL) were measured spectrophotometrically, using enzymatic colorimetric assay kits (Randox, Northern Ireland) using standard methods while low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) were calculated. The westergren method was used for Erythrocyte sedimentation Rate assay, Clot Weight Method for plasma fibrinogen assay, haemorheology was carried out using an LS30 Viscometer(Contraves AG, Zürich, Switzerland) for plasma viscosity assay, Neutrophil-Leucocyte Ratio was defined as the Log E Neutrophil Count/Log E Lymphocyte count within the peripheral blood. Serum antioxidant estimation was done using modified epinephrine assay method for SOD, spectrophotometric assay method for CAT estimation, GPx activity was measured using assay kits and MDA estimation was carried out using thiobarbituric acid assay. Data obtained from the biochemical assays were presented as mean ±SEM and analysed for statistical significance by one-way ANOVA using graph pad prism statistical software version 8.1 and probability value of p<0.05 was regarded as significant. In conclusion, the result of this study shows that high fat diet has no tendency to induce oxidative stress probably due to duration of consumption of high fat diet.Kolaviron on the other hand also caused a significant reduction in ESR and MDA, thus, is a potential anti-inflammatory and anti-oxidative agent.