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ABSTRACT
Jatropha tanjorensis is a plant that is known to possess various bioactive compounds with potential medicinal properties. Investigating its role in haemopoiesis and the associated molecular pathways could offer useful insights into its therapeutic potential for conditions related to blood cell synthesis and their related disorders. The objective of this study was to determine the effects of aqueous Jatropha tanjorensis leaf extract on RUNX1 mRNA and ETV6 mRNA in mediating haemopoiesis in male albino Wistar rats. A total of thirty (30) male adult albino wistar rats were selected into five (5) groups. The groups were control group, phenyl hydrazine group, 1000mg/Kg of Jatropha tanjorensis group, 2000mg/Kg of Jatropha tanjorensis group and 4000mg/Kg of Jatropha tanjorensis group. RNA extraction and semi-quantitative polymerase chain reaction (PCR) was used to isolate RNA from rat bone marrow, complimentary DNA was synthesized and was subsequently amplified using polymerase chain reaction. Gel electrophoresis was used to determine the bands of the genes. The findings revealed that the mRNA expression pattern of RUNX1 was significantly higher (p<0.001) in the untreated,1000mg/kg and 2000mg/kg when compared to the control group. The expression pattern of RUNX1 was significantly higher in 4000mg/kg group when compared to control group(p<0.05). The expression pattern of RUNX1 was significantly higher in groups treated with 1000mg/kg, 2000mg/kg and 4000mg/kg when compared to untreated group (p<0.05). The mRNA expression pattern of ETV6 was significantly lower (p<0.001) in untreated, 1000mg/kg and 4000mg/kg groups when compared to the control group. Among the treated groups, only 2000mg/kg showed a significantly higher (p<0.05) expression pattern of ETV6 when compared to both untreated 1000mg/kg groups. In conclusion, Jatropha tanjorensis significantly increased RUNX1 and ETV6. These results suggest that Jatropha tanjorensis could influence haemopoiesis by modulating the expression of the key regulatig factor.
