DETERMINATION OF THE ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ETHYLACETATE PULP EXTRACT OF AZANZA GARCKEANA

ABSTRACT

This experimental work was conducted to ascertain the antioxidant and antibacterial capabilities of Azanza garckeana ethylacetate pulp extract as well as to assess the phytochemical profiles and pharmacological characteristics of Azanza garckeana ethylacetate extracts. Fresh Azanza garckeana leafs, fruits and roots were collected from Tula in Kaltungo Local Government Area(LGA) of Gombe State, Nigeria on the 26th September 2019 and the study was carried out at the Department of Biochemistry, University of Benin, Benin city, Nigeria from January 2022 to December 2022. Fruit pulps from Azanza garckeana were dried by air to eliminate moisture. The pulp was then crushed with a mortar and pestle to create powder. The ground-up pulp was kept at room temperature in an airtight container until they were retrieved. The 200g of ground-up pulp was steeped in ethyl acetate for 72 hours while being agitated daily. The mixture went through a muslin cloth filter. In order to obtain the extract, the filtrate was freeze-dried. The ethyl acetate extract was kept at 4°C in the refrigerator prior to antioxidant and antibacterial analysis. The inhibition percentage of different concentrations of Azanza garckeana 's ethyl-acetate pulp extract is shown in the in vitro antioxidant activities of the extract. The percentage of inhibition increased with extract concentration in a non-significant (p>0.05) way, but sample E showed stronger activity than sample A did. The Ethyl acetate Pulp extract of A. garckeana exhibited poor FRAP, when compared with ascorbic acid (p < 0.05). The A. garckeana ethyl acetate pulp extract did not exhibit any Antibacterial activity and did not specifically inhibit any of the tested bacteria. The pulp of Azanza garckeana may have had an antioxidant effect in this study, but it's possible that a single concentration was what caused the Non-Antibacterial inhibitory activity.