DETECTION AND ISOLATION OF SAMONELLA FROM SACHET WATER IN UGBOWO, BENIN CITY, EDO STATE

ABSTRACT

Water is very essential to man’s daily survival. The availability of good quality water sources is becoming limitedas a result of contamination and water-borne diseases that poses several health issues. In this study, we aimed to investigate the presence of Salmonella species in local sachet drinking water, which is widely consumed in Ugbowo, Benin city.A total of five (5) sachet drinking water samples were collected from five water producing brands in Ugbowo, Benin City which consists; Notre dame table water, Uncle Sam water, Uniben water, Olivia water, and Oja table water. The samples were collected in duplicates from each shops. Samples were processed using standard microbiological techniques, including enrichment culture, selective media, and biochemical tests.Initial screening revealed the presence of colonies with morphological characteristics consistent with Salmonellaspecies in several samples. The highest mean bacterial count was obtained from Oja table water while the least count was obtained from Olivia water. All isolates were subjected to antibiotics susceptibility and resistance test with antibiotics such as Gentamycin, Aminoglycoside, Ciprofloxacin, Clindamycin and Augumentin. All isolates were resistant to antibiotics like Erythromycin, Carbapenem and Chloramphenicol. The findings from this study was in conformity to previous research in literature, highlighting variations in bacterial counts, antibiotic susceptibility patterns, multiple antibiotic resistance index and pathogenicity profiles among different water brands.