DESORPTION OF LACTOBACILLUS REUTERI, LACTOBACILLUS GASSERI AND LACTOBACILLUS RHAMNOSUS

ABSTRACT

Lactobacillus could exert beneficial effect on humans by inhibiting invasion of pathogens, improving the epithelial barrier function, modulating host immune system by adhering to these surfaces. Adhesion ability is regarded as an important property of probiotics. When probiotics adhere to the epithelium, they can function stably in the intestine. There are so many in-vivo and in-vitro methods for de-adhesion of organisms from surfaces. In this study, there was an evaluation on the best concentration of dimethyl sulphoxide that could desorb Lactobacillus reuteri, Lactobacillus gasseri and Lactobacillus rhamnosus from different adsorbents. Gram staining and micrometry revealed the organisms as gram positive with dimensions 5.4 µm and 1.68 µm, 5.4 µm and 1.10 µm and lastly 1.60 µm and 1.60 µm respectively. The growth inhibitory effect of dimethyl sulphoxide was then studied which made us know that the solubilizing agent was safe for the Lactobacillus species. The dimethyl sulphoxide at 25% desorbed most of the Lactobacillus reuteri from cotton wool surface. It was also able to desorb Lactobacillus gasseri and Lactobacillus rhamnosus from cotton wool surface best at 50% respectively. So, cotton wool was the best adsorbent that eluted the Lactobacillus species. Keywords: Probiotics, Lactobacillus, Adhesion, De-adhesion