ABSTRACT

Disinfection is a critical component of infection control, particularly in healthcare settings and food processing industries, where these bacteria pose significant threats to public health.This comparative study aimed to assess the efficacy of various disinfectants against two clinically significant bacterial pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. A range of commonly used disinfectants, including quaternary ammonium compounds, chlorine-based agents, hydrogen peroxide, and alcohol-based solutions, were evaluated for their effectiveness against P. aeruginosa and S. aureus. The study employed standardized methods to determine the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) for each disinfectant against both bacterial strains. In addition, time-kill assays were conducted to assess the disinfectants' bactericidal activity over varying exposure durations.

The results revealed notable variations in disinfectant efficacy between the two bacterial species. P. aeruginosa exhibited greater resistance to certain disinfectants, while S. aureus was more susceptible to specific agents. Notably, some disinfectants demonstrated rapid bactericidal activity against one bacterium but required extended contact times for effective elimination of the other. These findings emphasize the importance of tailoring disinfection protocols to the specific pathogens encountered, as a one-size-fits-all approach may not provide optimal results. Furthermore, they underscore the necessity for continuous monitoring of disinfection practices, especially in healthcare settings, to mitigate the risk of healthcare-associated infections. There is need for evidence-based selection and application of disinfection agents to effectively control these pathogens and ensure public health safety.