ABSTRACT
Polyherbal remedies have been suggested as a more economical and safer option for potential stress relief. The aim of this study is to evaluate the chronic anti-stress activity of polyherbal-formulated tea (Citrus limon, Curcuma longa, Zingiber officinale, Allium sativum, and Moringa oleifera). Chronic stress was induced in mice for twenty-one days using various stressors such as forced swimming endurance test, tail suspension test, food deprivation, water deprivation, cold restraint stress test, physical restraint test, acetic acid induced pain stress, animal isolation, heat exposure, introduction of mice into beddings of predator, and introduction of predators into mice cages. The animals were sacrificed, blood was collected for cortisol analysis, and the brain was homogenised and used to analyse for antioxidants such as superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase, as well as stress biomarkers such as cortisol and malondialdehyde.
