BLOOD PH AND PLASMA VISCOSITY OF SICKLE CELL ANEMIA IN CRISIS AND STEADY STATE

ABSTRACT

Sickle cell anemia (SCA) is a genetic blood disorder characterized by the presence of abnormal hemoglobin molecules in red blood cells. It is caused by a point mutation in the B-globin gene, resulting in the production of hemoglobin S(HbSS) instead of the normal hemoglobin A(AA). The aim of this study was to measure plasma viscosity and blood pH in Sickle cell anemia in crisis and steady state.90 human subject were used in the experiment,30 of which were Sickle cell subjects (HbSS) in crisis state, 30 of which sickle cell (HbSS) in steady state and the other 30 were used as normal hemoglobin control (HbAA). Blood was drawn from all 90 subjects and each blood sample was carefully analyzed for plasma viscosity using the Reid and Ugwus method, while the pH was analysed using a pH meter. Statistical analysis was done using Graph pad prism version 8.1, ap value of 0.05 was taken as statistically significant. The result showed a significant reduction in the pH and Viscosity in the subject in crisis compared with the steady state and control. In conclusion vaso-occlusive crisis results in increased blood acidosis (reduced pH) and reduced viscosity.