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ABSTRACT
The goal of this research is to examine genotoxicity of cassava effluent on the gills of Freshwater clam [Egera radiata LAM (BIVALVIA, DONACIDAE)] using the comet assay (single cell gel electrophoresis) method. The clam was exposed to sub-lethal concentrations (0.1, 0.5, 1.5, 2.5%) of the cassava effluent for 96hours and a control, and the tissue samples from gills were analyzed using the comet assay. The result gotten form the comet assay revealed a predominant significant damage in the gills which were dose dependent compared to the control. Comet assay is many times associated with other techniques, offering good results to increase efficiency in environmental assessment or to evaluate genotoxicity of pollutants individually. Excessive agricultural and industrial activities adversely affect biodiversity, threatening the survival of species in a particular habitat, as well as posing disease risks to humans. Comet assay is a quick, sensitive, and low-cost technique for detecting DNA strand breakage.
