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ABSTRACT
Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries and natural water bed. The aim of this work was to determine the microbial content present in freshwater bivalves. Proximate analysis was carried out using standard chemical techniques. Microbial count was done using the pour plate method. Antimicrobial susceptibility test was also carried out using the agar well diffusion method. Using chitosan as an antimicrobial agent, the concentrations used were 5.80mg/ml, 2.90mg/ml, 1.45mg/ml and 0.75mg/ml. Enteric bacteria isolates found in freshwater bivalves include Escherichia coli, Klebsiella pneumonia, Yersinia sp, and Proteus sp. The bacterial count on nutrient agar, MacConkey agar, and eosin methylene blue agar were 2.48 x 104 cfu/ml, 4.55 x 104 cfu/ml, and 24.80x 104 cfu/ml respectively. No effect was recorded on Escherichia coli and Enterobacter aerogenes in all concentrations (0.00±0.00mm) while Klebsiella pneumoniae and Yersinia pestis showed more inhibition by the various concentrations of chitosan with 3.53±0.18mm and 3.20±0.42mm as the respective zones of inhibition.
