You have no items in your shopping cart.
ABSTRACT
Monosodium glutamate (MSG), is a widely used food additive that enhances the savory flavor in numerous culinary applications. MSG administrations has been reported to induce oxidative stress in the blood serum. The aim of this study was to determine the bacteriological and heamatological profile of laboratory rats fed with Monosodium glutamate (MSG) supplemented fed. Monosodium based seasoning (Ajinomoto) was purchased from Oba market, Benin City. A total of 10 rats were used in this study, 5 were fed with MSG-supplemented feeds for 14days and 5 were control. The weight of the rats were monitored for 3weeks while stool and blood samples were obtained using sterile sample tubes/bottles. Stool samples were analyzed using basic microbiological while blood samples were analyzed for hematological profile using automated hematology analyzer. Antibiotics susceptibility profile of the bacteria isolates were determined using Kirby-Bauer disc diffusion method. Bacteria isolates were screened for the presence of phenotypic virulence characteristics such as hemolysin, gelatinase and lipase production. Bacteria counts obtained from samples ranged from 1.55x106 ± 2.00 to 3.11x106±3.50. The isolated bacteria include Staphylococcus aureus, Streptococcus pyogenes, Lactobacillus sp, Escherichia coli, bacillus cereus and Pseudomonas sp. Staphylococcus sp. (16.67%) was the most occurring bacteria isolates while Escherichia coli (8.3%) was the least occurring. Results of phenotypic virulence characteristics showed that most of the isolates were negative to gelatinase and lipase while results of the hemolysin test showed that most isolates expressed γ-hemolytic while Staphylococcus sp. and Staphylococcus aureus showed β-hemolysis. The hematological profile of rats showed a decrease in the concentration of white and red blood cells, lymphocytes, granulocytes and an increase in platelets counts. Most isolates showed susceptibility to antibiotics such as Erythromycin, Ciprofloxacin, Gentamycin and Pefloxacin. MSG consumption may influence the growth of bacteria in the gut microbiota, potentially altering metabolic processes, nutrient absorption and overall gut health.
