ABSTRACT
Large volumes of wastewater containing a complex mixture of organic and inorganic compounds are generated by the brewing industry, which is a major contributor to industrial effluent pollution. Brewery effluent contains high concentrations of organic matter, nutrients, and potentially harmful microorganisms, among other pollutants that, if improperly treated, could seriously harm the environment and public health. Therefore, determining the impact of brewery effluent on the environment and public health as well as developing efficient treatment plans to lessen its negative effects depend heavily on the bacteriological analysis of the effluent. In order to characterize the composition of the microbial community, determine whether pathogenic bacteria are present, and determine whether the current wastewater treatment procedures are effective in reducing microbial contamination, this project will perform a thorough bacteriological analysis of brewery effluent. Effluent from various brewing stages, including raw wastewater, primary treatment, secondary treatment, and final effluent discharge points, will be sampled for the study. Escherichia coli, fecal streptococci, total coliforms, fecal coliforms, and other indicator bacteria frequently linked to fecal contamination are among the bacteriological parameters that will be evaluated. Furthermore, nextgeneration sequencing (NGS) and polymerase chain reaction (PCR) are sophisticated molecular techniques that will be used to identify and quantify particular bacterial species—including potentially pathogenic strains—present in the effluent samples. In addition, the project will use microbial source tracking (MST) studies to distinguish between bacterial populations that originate from the brewery and those that originate from other sources in order to study the effects of brewery effluent discharge on receiving water bodies. This will offer important new information about how brewery effluent contributes to overall microbial pollution in aquatic environments. It is anticipated that the results of this investigation will enhance our comprehension of the microbial dynamics present in brewery effluent and their consequences for the environment and public health. Furthermore, the findings will help safeguard ecosystems and water quality by informing the creation of sustainable and more effective wastewater treatment methods that are specific to the microbial makeup of brewery effluent.
