Assessment of the Antioxidant and Antibacterial activities of ethyl acetate seed extract

ABSTRACT

Azanza garckeana. is a crucial dietary and therapeutic herb that has been employed throughout tropical Africa. Therefore, this study aimed to assess the antioxidant and antibacterial activities of ethyl-acetate seed extract of Azanza garckeana. The seeds of Azanza garckeana were air dried, pulverized and extracted using ethyl-acetate as the solvent. The seed extract was subject to antioxidant assays using DPPH and FRAP assay method and antibacterial evaluation using agar diffusion and microdilution assays. The result reveals increasing percentage inhibition of DPPH activities with higher concentration and reduced FRAP capacity in comparison with standard ascorbic acid. In-vitro antibacterial study using test organisms such as E. coli, P. Aeruginosa, S. aureus, Streptococcus spp., Klebsiella spp and P. mirabilis, shows that seed extract exhibited a high zone diameter of inhibition in Streptococcus spp and S.Aureus. The extract also do not have minimum inhibitory concentration activity in all the test organisms. Thus, the present study provides insights into the antioxidant and antibacterial activity of Azanza garckeana, and its potential to mop out free radicals.