You have no items in your shopping cart.
ABSTRACT
The increased human activities at the motor park lead to the proliferation of airborne bacteria. β lactam Drugs are commonly recommended for the treatment of infections caused by Enterobacteriaceae. The study was aimed at assessing airborne bacteria and extended spectrum β-lactamase (ESBL) producing enterobacteriaceae isolated from the motor parks in Benin City. Airborne bacteria samples were collected in Central, Ugbowo, Agip, Nineteenth Street and Ekosodin parks, in Benin City using the settled plate method on Nutrient, MacConkey and blood agar. Temperature, relative humidity and pressure were measured. Characterizations of bacterial isolates were done using standard microbiological techniques. Antibiotic sensitivity profile was investigated by disc diffusion method. ESBL detection and Confirmation using CDT was investigated. Virulence factor analysis was studied on ESBL producers. The molecular basis of resistance was achieved by plasmid profiling and curing techniques and the detection of genes encoding β-lactamase enzyme of the ESBL producers was carried out using appropriate primers. The temperature, relative humidity and atmospheric pressure at the point of agar plate exposure ranged from 25.9 to 29.8 oC, 70 to 90 % and 1008 to 1014 N/m2 respectively. The bacterial load on nutrient agar ranged from 0.84±0.63×103 to 1.40±0.04×103 cfu/m3 . The coliform load on MacConkey agar ranged from 0.19±0.48×103 to 0.50±0.41×103 cfu/m3 , and blood agar from 0.80±0.48×103 to 1.17±0.12×103 cfu/m3 . Enterobacteriaceae constitutes 56% of the total bacterial isolated. The highest and lowest isolates with frequency of occurrence were Escherichia coli and Citrobacter diversus with values 29.05% and 1.42% respectively. Klebsiella pneumoniae recorded the highest MAR index of 0.8 before plasmid curing. Virulent factor analysis of ESBL producers showed that Proteus mirabilis, and Serratia marcescens were 60%. Serratia marcescens possessed all the genes TEM, CTX-M, SHV and OXA. Environmental sanitation, reduced overcrowding, effective waste disposal at the parks are essential to reducing the proliferation of ESBL producing airborne bacteria.
