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ABSTRACT
Citullus colocynthis is a member of the cucurbitacea family, its seed are well known for their numerous medicinal purpose. This research work was done to screen the activity of pulp and seed of Citrullus colocynthis against selected bacteria isolates. The microorganisms isolated include Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Escherichia coli. Isolates were further identified based on their cultural, morphological and biochemical characteristics to ascertain their identity, water extract was used for antibacterial assay. Phytochemical properties of the extract showed the presence of primary metabolite (carbohydrate, protein) and secondary metabolite (flavonoids, saponins). Extract from pulp, concentration determined were 100 mg/ml, 75 mg/ml, 50 mg/ml, 25 mg/ml and 12.5 mg/ml. No effect were recorded on all bacteria isolates at 25 mg/ml and 12.5 mg/ml concentration while Staphylococcus aureus and Escherichia coli were more inhibited at various concentration (25 mg/ml and 12.5 mg/ml respectively). Extract from seed antibacterial effect were not recorded on all bacteria isolates at (50 mg/ml, 25 mg/ml and 12.5 mg/ml) while Staphylococcus aureus and Escherichia coli were more inhibited at 100 mg/ml concentration. The minimum inhibitory concentration values ranging from 50mg/ml against E. coli to 100mg/ml against P. aeruginosa in pulp extract while in seed extract the MIC values ranges from 75mg/ml against S. aureus to 100mg/ml against E. coli. The inhibitory effects of this extracts were compared with standard antibiotics ciproflaxin. Results indicated that aqueous extracts has a similar inhibitory effect with ciproflaxin.
