ABSTRACT
This study aimed to evaluate the antioxidant properties, total phenolic content (TPC), and total flavonoid content (TFC) in the methanol extract of Alchornea cordifolia leaves. TPC and TFC were quantified, and the antioxidant capacity of the plant extract was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay as well as Total Antioxidant Capacity (TAC). To determine TPC, the Folin-Ciocalteu colorimetric method was employed, using gallic acid as the standard, and the absorbance of various extract concentrations was measured at 750 nm. The leaves underwent phytochemical screening, revealing the presence of phenols, flavonoids, alkaloids, glycosides, eugenols, steroids, terpenoids, and carbohydrates, with the absence of tannins. The study found that the Total Phenolic Content (TPC) was measured at 106.10 ± 1.98 mg GAE/g, and the Total Flavonoid Content (TFC) was determined to be 84.55 ± 3.34 mg QE/g. In the DPPH assay, the IC50 value for the standard ascorbic acid was 1.32 μg/mL, while for the extract, it was 2.99 μg/mL. As for the Total Antioxidant Capacity (TAC), the IC50 was 221.6 μg/mL for ascorbic acid and 246.8 μg/mL for the methanol extract of the leaves.These results underscore the potential of Alchornea cordifolia leaves as a valuable source of natural antioxidants, suggesting their significance in the field of medicinal applications.
