ANTIMICROBIAL STUDY, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT

ABSTRACT

Justicia secunda leaves were analyzed for the total phenolic and flavonoid content. The phenolic content was determined using folin-Ciocalteu assay. The absorbance of total phenolics and total flavonoids were measured at wavelength of 750nm and 415 nm respectively using the UV-visible spectrophotometer. The results revealed a total phenolic content of 12.83 ± 0.97 mg GAE/g extract (expressed as Gallic equivalent) and total flavonoid content of 21.03 ± 1.71 mg QE/g extract (expressed as quercetin equivalent). Consequently, validates the use of Justicia secunda in managing oxidative related ailments as a result of its rich antioxidant properties. The methanol extract of Justicia secunda leaves had no bactericidal effect on all four concentrations (25 mg/mL, 50 mg/mL, 75 mg/mL, 100 mg/mL) on Proteus vulgaris as the concentration was completely resistant. Methanol extract of Justicia secunda leaves had bactericidal effect on all four concentrations (25 mg/mL, 50 mg/mL, 75 mg/mL, 100 mg/mL) on Pseudomonas aeruginosa and Escherichia coli and were seen to be sensitive on the four concentrations. Staphylococcus aureus was seen to be sensitive on 25 mg/mL, 50 mg/mL, 75 mg/mL concentrations and resistant at 100 mg/mL.