ANTIMICROBIAL EFFECT OF THE LEAF EXTRACT OF Bryophyllum pinnatum ON SOME CLINICAL ISOLATES

ABSTRACT

Antibiotic resistance is on the rise among bacteria, necessitating the discovery of new and safer antimicrobials, particularly from natural sources such as plants. The leaves of Bryophyllum pinnatum are one of many plants that rural people have used to treat ailments. The antibacterial effect of Bryophyllum pinnatum leaf extract on three bacteria species, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, was investigated experimentally using the Agar well diffusion method, which was prepared with methanol, ethanol, and aqueous solution, respectively. We discovered that methanol and ethanol extracts were more sensitive to the strains than aqueous solution based on the data collected at the end of the experiment. The four distinct wells where the extracts were poured into were made using four different concentrations of 100, 75, 50, and 25. The antibacterial action of ethanol was found to be the strongest of the three solvents examined. The zone of inhibition of the isolates by aqueous methanol and ethanol crude extract is shown in Table 1-3. The Minimum Inhibitory Concentration is shown in Tables 4 and 5. The Minimum Bactericidal Concentration is shown in Tables 6 and 7. The antibiotic sensitivity test is shown in Table 8-10.