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ABSTRACT
A major problem in Nigeria has been the management of waste, its adequate treatment alongside its flow. Hence this research was conducted to investigate the antimicrobial activity, proximate and phytochemical analysis of bitter leaf and pumpkin leaf cultivated in soil watered with beauty hair salon wastewaters. Salon wastewater samples were collected from Pee Beauty Place comprising of six (6) hair salon shops, located in Benin City, Edo state, Nigeria. Stems of bitter leaf (Vernonia amygdalina) and the seeds of pumpkin (Telferia occidentalis) were purchased from local market in Benin City. The seeds and stems were planted in pot containing soil at a depth of 2- 3cm deep. The pots were watered daily with 1400ml of tap water for the control pots and 1400ml of salon wastewater for treatment pots for 7 days before planting and watering continued after planting. The leaves of mature plant samples were cut into smaller pieces and air dried for 4weeks at ambient temperature. Two hundred grams (200g) of each pulverized plant sample was weighed into separate extracting bottle and was cold extracted in 1 litre of ethanol reagent for three days (72hrs). Plant filtrate was analysed for antimicrobial activity while leaf samples were tested for micronutrient and phytochemical compositions.
