ANTIBACTERIAL ACTIVITIES OF HONEY PRODUCED BY Apis mellifera On Escherichia coli, Staphylococcus aureus.

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ABSTRACT

Honey is produced by Apis mellifa and it is used for many purposes such as for burns, herpes, diabetes, cancer, wounds and ulcers. The antibacterial activity of honey bought from Ring road was carried out using the disk diffusion method. The disk were prepared using the filter papers and were sterilized, several dilution was carried using sterile distilled water up to 10-3. The organism were obtained from University of Benin Teaching Hospital and were re-identified using standard cultural morphological characteristics. Biochemical tests were also used to identify the isolates.

This study was done to investigate the antibacterial activity of Honey produced by Apis mellifera on Escherichia coli and staphylococcus aureus which are generally resistant to most antibiotics currently in use. The concentration use were 100%, 10-1, 10-2, and at 10-3. The highest zone of inhibition for was 14.0mm while the lowest was 2.0. At 100% concentration, the samples showed antimicrobial effects on all the test organisms. However, a reduced effect was seen following the serial dilution of the sample. The result obtained from this study indicates that honey can be used as type of antimicrobial agent.

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