ANTI-MICROBIAL ACTIVITY OF HEXANE EXTRACT OF Anthocleista djalonensis

ABSTRACT

Phytochemicals have a wide range of biological activities ranging from antioxidants to antimicrobial. This study was aimed at examining the anti-microbial effect of hexane extract of A. djalonensis. The methodology involved isolation collection, on determination of microbial phytochemical properties. Also, confirmatory tests were carried out to determine the cultural and morphological characteristics of the isolates. The microbial isolates used in this study were Enterobacter cloacae, Serratia sp, Bacillus sp and Vibrio. CLSI methods was used in anti-microbial analysis of A. djalonensis extract against some selected isolates. The results showed antibacterial effects on Enterobacter cloacae with zones ranging from 0.00±0.00 to 12.00±1.00mm, the extract also had antimicrobial effect on Vibrio sp with zones ranging from 0.00±0.00 to 18.00±2.00mm. Serratia sp and Bacillus sp had no inhibitory activity. The fungi isolates used in this study were Aspergillus niger and Penicillium sp. The zones of inhibition for Penicillium ranged from 8.00±0.00mm while Aspergillus ranged from 0.00±0.00 to 8.00±0.00mm. The minimum fungicidal concentration for both isolates was 75mg/ml. The hexane extract of A. djalonensis demonstrated significant inhibitory effects against these microorganisms, indicating its potential as a natural anti-microbial agent. The choice of hexane as an extraction solvent for A. djalonensis is supported by its effectiveness in extracting bioactive compounds with antimicrobial properties, its low toxicity profile, and its ability to penetrate cell membranes efficiently.