ANTI DIABETIC AND GLUCOSE TOLERANCE EFFECT OF THE BI-HERBAL LEAF AQUEOUS EXTRACT ON STREPTOZOTOCIN INDUCED DIABETES IN WISTER RATS

ABSTRACT

Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. It is rapidly increasing worldwide, posing significant concerns in both developed and developing countries. The current oral anti-diabetic medications have limitations in terms of efficacy and safety. This, coupled with the global epidemic of diabetes mellitus, has led to the rising popularity of alternative therapies for managing the disease more effectively and safely. This study evaluated the anti-diabetic activities of bi-herbal extract in streptozotocin-induced diabetic rats. The plants were collected, authenticated, and processed into aqueous extracts. Thirty-six adult male Wistar rats were used for the study, with diabetes induced using streptozotocin. Following treatment with different doses of the bi-herbal extract (50, 100 and 200 mg/kg), blood glucose levels were measured using a glucometer, and the random glucose tolerance test was conducted which assessed the glucose tolerance.Doses of 100, and 200 mg/kg significantly reduced blood glucose levels compared to the control groups. Furthermore, the study found that a bi-herbal extract at a dose of 100 mg/kg body weight had a significant decrease in blood glucose levels (p <0.05). These findings underscore the promising anti-diabetic effects of the bi-herbal aqueous extract by improving glucose tolerance and lowering blood sugar levels. Further investigations are warranted to elucidate its long-term effects and potential mechanisms of action.