ABSTRACT
Herbal formulations, sometimes known as "polyherbal formulations," have drawn considerable interest. The aim of this study is to evaluate the acute anti-stress activity of poly-herbal-formulated teas (Curcuma longa, Moringa oleifera, Zingiber officinale Roscoe, Citrus limon, Allium sativum, xii and Syzygium aromaticum). This study was evaluated using a tail suspension test, a force swimming test, and a cold restraint stress test. In the tail suspension test, the polyherbal-formulated tea at 5 mg/kg, 10 mg/kg, and fluoxetine at 20 mg/kg significantly reduced the time of immobility when compared to the stress control. (***p <0.001; **p <0.01; *p <0.05). In the forced swimming test, the polyherbal-formulated tea at 10 mg/kg and fluoxetine at 20 mg/kg increased swimming time when compared with stress control. (**p <0.01; *p <0.05). In the cold restraint test, the polyherbalformulated tea at 5 mg/kg, 10 mg/kg, and fluoxetine at 20 mg/kg reduced the cortisol level in mice when compared to stress control. (*p <0.05; *p <0.05). The polyherbal-formulated tea at 5 mg/kg and 10 mg/kg significantly increased the superoxide dismutase levels in mice when compared to the stress control. (****p <0.0001; ***p <0.001). At 10 mg/kg, polyherbal-formulated tea and fluoxetine (20 mg/kg) increased catalase levels, and at 5 mg/kg and 10 mg/kg, polyherbalformulated tea and fluoxetine (20 mg/kg) significantly reduced the malondialdehyde (MDA) level in the animals when compared to stress control (*p <0.05; *p <0.05). In conclusion, the polyherbalformulated tea possesses antistress properties.
