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ABSTRACT
Environmental pollution caused by petroleum and petrochemical products has attracted much attention in recent decades, bioremediation remains one of the potential means to reclaim petroleum hydrocarbon contaminated soil. The objective of this study was to examine the levels of acid and alkaline phosphatase activity in petroleum hydrocarbon contaminated soil remediated with cassava mill effluent after six months. In the experimental procedure, sample 6-10 were polluted with 500ml of the pollutant (waste engine oil), sample 1 acted as the general control, sample 6 served as the pollution control, sample 2-5 served as the treatment control while sample 7-10 were the polluted and treated samples, the study continued for six months. The determination of Acid and Alkaline phosphatase activity was carried out spectrophotometrically at 405nm. The result obtained showed that there was no significant difference in the level of acid phosphatase in the polluted samples and the control, hence the cassava mill effluent maintained the levels of acid phosphatase activity in the soil. In the result obtained for the activity of alkaline phosphatase, there was a significant increase in the level of the enzyme activity in the polluted samples compared to the control. In respect to the enzymatic factors, cassava mill effluent has the potential to be used as a bioremediation agent.
