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Enzymes are biological catalyst and are protein in nature with an exception of ribozyme, an RNA catalyzing the RNA splicing in eukaryotes. Almost all metabolic processes in living system require enzymes to function. They are catalytically active in isolated forms. Living cells are not essential to get the enzyme activity. This property of enzymes has been exploited by the biochemists to study enzymes 'in vitro'.
Although, mostly enzymes are found within the cells, there are few enzymes especially in microbes which are secreted in the medium by the microbes and are called extracellular enzymes.
