A COMPARATIVE STUDY ON THE IN VITRO ANTIOXIDANT ACTIVITY ON AQUEOUS AND HYDROETHANOL EXTRACT

ABSTRACT

This study aimed to assess and compare the antioxidant potential of the aqueous and hydroethanol extract of a polyherbal formulation comprising Hibiscus Sabdariffa, Syzygium Aromaticum, Zingiber Officinale, and Cinnamomum Verum. Specifically, the study sought to determine whether the plant extract serves as a rich source of antioxidants and to investigate if the combined extracts exhibit synergistic antioxidant effects or potential interactions that may enhance their individual antioxidant activities. Tests for total antioxidant capacity (TAC), 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and ferric reducing power (FRAP) were performed in vitro. The obtained results from the FRAP AND DPPH Assays showed that the studied extracts had significant antioxidant capability. In terms of Antioxidant Capacity, it was observed that the Hydro-ethanol extract had a higher value compared to that of the Aqueous extract. The polyherbal formulation extracts demonstrated significant antioxidant capacity, highlighting its potential relevance as a source of natural antioxidants for numerous applications, the potential benefits of the polyherbal formulation as a source of enhanced-effective natural antioxidants are highlighted by this study. The study looked at whether the combined effects of the polyherbal extracts resulted in increased antioxidant activity when compared to the individual components, and it revealed a synergistic enhancement in its antioxidant activity. However, the mechanisms responsible for this synergy remains unknown and demand more study.